在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1504ZR-75-30 人乳腺癌細(xì)胞

ZR-75-30 人乳腺癌細(xì)胞

簡要描述:CRL-1504 ZR-75-30 人乳腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1504
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1995

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1504  ZR-75-30 人乳腺癌細(xì)胞

ATCC® Number:CRL-1504™Price:
Designations:ZR-75-30Depositors:LW EngelBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: mammary gland; breastDisease: ductal carcinomaDerived from metastatic site: ascitesCell Type: epithelialPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-1504  ZR-75-30 人乳腺癌細(xì)胞DNA Profile (STR):Amelogenin: XCSF1PO: 10,11D13S317: 10,11D16S539: 9D5S818: 12,13D7S820: 11,12THO1: 8,9TPOX: 10,11vWA: 18,19Cytogenetic Analysis:This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 40% of cells. The rate of cells with higher ploidies was 1.2%. Sixteen marker chromosomes were found in most cells.Age:47 yearsGender:femaleEthnicity:BlackComments:ZR-75-30 was derived from malignant ascites fluid from a 47-year-old premenopausal Black woman with infiltrating ductal carcinoma. These cells have been characterized to be human, non-HeLa, malignant mammary epithelium in origin.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommendedMedium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseDoubling Time:110 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020purified DNA:ATCC 45532purified DNA:ATCC 45533purified DNA:ATCC CRL-1504DReferences:1230: Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 21219323031: Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 68822532275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764


CRL-1504  


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久天天躁狠狠躁夜夜不卡| 丰满少妇在线观看网站| 日本爽快片18禁免费看| 两个女人互添下身视频在线观看 | 国产免费人成在线视频| 欧美黑人又粗又大又爽免费| 色偷偷偷久久伊人大杳蕉| 国产美女av一区二区三区| 欧美日韩午夜群交多人轮换| 午夜精品久久久久久久99老熟妇| 国产欧美在线一区二区三区| 亚洲熟妇无码av另类vr影视| av无码久久久久不卡免费网站| 国产成人精品三级麻豆| 国产三级a在线观看| 无码熟熟妇丰满人妻啪啪| 精品综合久久久久久98| 欧美xxxx色视频在线观看| 日韩精品射精管理在线观看| 免费在线高清亚洲av| 国色天香社区视频在线| 妺妺窝人体色www聚色窝| 久久天天躁狠狠躁夜夜躁2014 | 日韩熟女精品一区二区三区| 国产精品99久久精品爆乳| 成人网站免费观看入口| 婷婷成人丁香五月综合激情| 精品国偷自产在线视频九色| 成在线人午夜剧场免费无码| 女人被狂躁到高潮视频免费网站| 激情欧美成人久久综合| 肉岳疯狂69式激情的高潮| 高清一区二区三区日本久| 黄色三级国产视频无毒| 国产精品热久久高潮av袁孑怡| 男女乱婬真视频| 亚洲熟妇色xxxxx亚洲| 欧美巨大黑人极品精男| aaa少妇高潮大片免费看| 孕妇怀孕高潮潮喷视频孕妇| 亚洲av日韩av无码污污网站|